中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (29): 4618-4624.doi: 10.3969/j.issn.2095-4344.2014.29.005

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

瘢痕疙瘩及正常皮肤中骨形态发生蛋白7、Gremlin、血管内皮生长因子、高迁移率族蛋白B1的表达

刘玉芳1，林 茂2，侯彬彬2，侯 娜1，刘 霞2，王 丹3，许雪珠2   

  1. 1大连大学附属中山医院皮肤科，辽宁省大连市 116001；大连医科大学附属第二医院，2皮肤科，3外科门诊手术室，辽宁省大连市 116023
  • 修回日期:2014-04-16 出版日期:2014-07-09 发布日期:2014-07-09
  • 通讯作者: 许雪珠，博士，教授，主任医师，硕士生导师，大连医科大学附属第二医院皮肤科，辽宁省大连市 116023
  • 作者简介:刘玉芳，女，1985年生，山东省潍坊市人，大连大学附属中山医院皮肤科在读硕士，主要从事皮肤外科方面的研究。
  • 基金资助:

    国家自然科学基金资助项目(81171489)

Expression of bone morphogenetic protein 7, Gremlin, vascular endothelial growth factor and high mobility group box-1 in keloid and normal skin

Liu Yu-fang1, Lin Mao2, Hou Bin-bin2, Hou Na1, Liu Xia2, Wang Dan3, Xu Xue-zhu2   

  1. 1Department of Dermatology, Zhongshan Hospital Affiliated to Dalian University, Dalian 116001, Liaoning Province, China; 2Department of Dermatology, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China; 3Outpatient Surgery Department, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China
  • Revised:2014-04-16 Online:2014-07-09 Published:2014-07-09
  • Contact: Xu Xue-zhu, M.D., Professor, Chief physician, Master’s supervisor, Department of Dermatology, Second Affiliated Hospital of Dalian Medical University, Dalian 116023, Liaoning Province, China
  • About author:Liu Yu-fang, Studying for master’s degree, Department of Dermatology, Zhongshan Hospital Affiliated to Dalian University, Dalian 116001, Liaoning Province, China
  • Supported by:

     the National Natural Science Foundation of China, No. 81171489

PDF

377

被引次数

2

摘要：

背景：瘢痕疙瘩的发生是创伤修复的纤维化过程，多种纤维化相关细胞因子参与其发生发展。骨形态发生蛋白7、Gremlin与高迁移率族蛋白B1在其他器官纤维化中发挥重要作用，而在瘢痕疙瘩组织中的研究少见报道。

目的：分析骨形态发生蛋白7、Gremlin(骨形成蛋白拮抗剂家族成员)、血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩发病机制中的作用和意义。
方法：采用免疫组织化学方法及Wsetern Blot法检测骨形态发生蛋白7、Gremlin、血管内皮生长因子与高迁移率族蛋白B1在20例瘢痕疙瘩组织和20例正常皮肤组织中的分布及蛋白水平的表达，并对瘢痕疙瘩组织中骨形态发生蛋白7与Gremlin、血管内皮生长因子与高迁移率族蛋白B1的表达情况进行相关性分析。

结果与结论：与正常皮肤组织相比，Gremlin、血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩组织中表达呈显著性上调(P < 0.01)，而骨形态发生蛋白7的表达呈显著性下调(P < 0.01)，Gremlin与骨形态发生蛋白7在瘢痕疙瘩中的表达呈显著性负相关(r=-0.539，P < 0.05)。血管内皮生长因子与高迁移率族蛋白B1在瘢痕疙瘩中的表达呈显著性正相关(r=0.560，P < 0.05)。说明Gremlin的过度表达和骨形态发生蛋白7的表达下调，以及高迁移率族蛋白B1与血管内皮生长因子的高表达可能共同参与了瘢痕疙瘩的纤维化病理过程。



中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

关键词: 组织构建, 组织工程, 骨形态发生蛋白7, Gremlin, 血管内皮生长因子, 高迁移率族蛋白B1, 瘢痕疙瘩, 纤维化, 国家自然科学基金

Abstract:

BACKGROUND: The development of keloid is a progress of fibrosis in wound healing, and involves various fibrosis-related cytokines. Bone morphogenetic protein 7 (BMP7), Gremlin and high mobility group box-1 (HMGB1) play an important role in fibrosis of many organs, but their role in keloid tissue has rarely been reported.

OBJECTIVE: To investigate the role of BMP7, Gremlin, vascular endothelial growth factor (VEGF) and HMGB1 in the development of keloid.
METHODS: The protein levels and distribution of BMP7, Gremlin, VEGF and HMGB1 in 20 cases of keloid and 20 cases of normal skin were detected by immunohistochemistry and western blot analysis, respectively. And the correlations among expression levels of BMP7, Gremlin, VEGF and HMGB1 in keloid were analyzed.
RESULTS AND CONCLUSION: In keloid tissue, the expression levels of Gremlin, VEGF and HMGB1 were significantly higher than that in normal skin (P < 0.01), while the expression levels of BMP7 were significantly lower (P < 0.01). The levels of Gremlin were negatively correlated with the levels of BMP7 (r=-0.539, P < 0.05). And the levels of VEGF were positively correlated with the levels of HMGB1 (r=0.56, P < 0.05). The overexpression of Gremlin and decreased expression of BMP7, as well as the increased expression of HMGB1 and VEGF, may contribute to the pathogenesis of fibrosis in the development of keloid.


中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程


全文链接：

Key words:  

中图分类号: